Mahlathini and the Mahotella Queens (also known as Mahlathini Nezintombi Zomgqashiyo - Mahlathini and the Girls of Mgqashiyo) was a South African mbaqanga supergroup composed of:

The late Simon 'Mahlathini' Nkabinde (1937–1999), a phenomenal singer in the basso-profundo 'groaning' style

The mgqashiyo session group Mahotella Queens (1964–present), known for their fast-paced dancing and multi-part harmonies - originally five members from 1964–1986, now a trio (1986–present)

The mbaqanga instrumental band Makgona Tsohle Band (1963–1999), led by the late Marks Mankwane and West Nkosi

In 1998, band member West Nkosi was killed in a road accident. Marks Mankwane died not long afterwards, followed by the death of Mahlathini in July 1999, thus ending the group's existence. Despite this, the Mahotella Queens recruited brand new (younger) musicians in a new group line-up and continue performing and recording to this day, having released successful albums such as Sebai Bai (2001), Bazobuya (2004), Reign & Shine (2005) and Siyadumisa (Songs of Praise) (2007).